Adjudin (AF-2364)|DC Chemicals

Adjudin (AF-2364)|DC Chemicals

AF-2364, an analogue of Lonidamine (LND), had a contraceptive effect when orally administered to adult Sprague–Dawley rats.

Product Name: Adjudin (AF-2364) |Catalog Number:  DC8355 | cas: 252025-52-8 | Other names:  | Chemical name：1-(2,4-dichlorobenzyl)-1H-indazole-3-carbohydrazide | Molecule Formula: C15H12Cl2N4O | MW: 335.19

AF-2364 or AdjudinTM, also known as 1-(2,4-dichlorobenzyl)-1H-indazole-3-carbohydrazide, is a less toxic but functionally similar analogue of Lonidamine (LND). AF-2364 has been shown to disrupt adherens junctions (AJs) between Sertoli cells and round and elongated spermatids in the adult rat and has been tested as a potential male contraceptive. In 2006, AF-2364 was conjugated to a recombinant follicle-stimulating hormone mutant protein, which served as its 'carrier' to Sertoli cells in the testis. This study is now moving towards human trials, though some major issues still need to be resolved. Recent research revealed a similar AF-2364-induced inhibition of spermatogenesis in the rabbit. However, no published studies have determined the effects of AF-2364 on human sperm function or fertility in general. As LND can inhibit oxygen consumption and block energy metabolism in tumour cells by targeting the mitochondria permeability transition (PT) pore and causing the loss of ΔΨm, we wondered whether AF-2364 would target human sperm mitochondria to influence sperm function and thus warrant further development into a spermicide candidate.

For research and scientific purpose only, not for human use.