N3PT|cas 13860-66-7|DC Chemicals

N3PT|cas 13860-66-7|DC Chemicals

N3PT(N3-pyridyl thiamine) is a potent and selective transketolase(TK) inhibitor (IC50= 22 nM for Apo-TK) both in vitro and in vivo.

IC50 Value: 22 nM( Apo-TK) ; 26 nM (EC50, Cellular TK)

Target: transketolase

in vitro:  N3PT inhibits transketolase activity in a cell based assay.

Product Name: N3PT|Cat No: DC9543|Cas: 13860-66-7|Molecule Formular: C13H19Cl2N3OS|Molecule Weight: 336.2805|Other names: N3PT

N3PT(N3-pyridyl thiamine) is a potent and selective transketolase(TK) inhibitor (IC50= 22 nM for Apo-TK) both in vitro and in vivo.
in vitro:  N3PT inhibits transketolase activity in a cell based assay. Competitive inhibition of TK by N3PT in cells treated with increasing doses of thiamine, expressed as percentage enzymatic activity (the slope of initial linear range) of controls not treated with compounds.
in vivo: Tumors were induced in mice at day 0 and mice were then treated at day 7 with vehicle alone or with N3PT.  Low-thiamine diet enhances the sensitivity to N3PT inhibition of TK in spleen. Animals were switched to diets containing 16.5 mg/kg (unchanged), 5 mg/kg, 1 mg/kg, or 0 mg/kg thiamine, from a normal chow containing 16.5mg/kg thiamine.

For research only, not for human use!