BTB-1|BTB1|cas 86030-08-2

BTB-1|BTB1|cas 86030-08-2

DC Chemicals, Website: www.dcchemicals.com

Product Name: BTB-1|Cas Number: 86030-08-2| Catalog Number: DC10164|Other Nmaes: BTB 1; BTB1

BTB-1 is a small molecule inhibitor of the mitotic motor protein Kif18A.

BTB-1 blocks the motility of Kif18A in a reversible manner. BTB-1 inhibits Kif18A in an adenosine triphosphate (ATP)-competitive but microtubule-uncompetitive manner and slows down the progression of cells through mitosis. 100 μM BTB-1 does not significantly inhibit any of the other tested mitotic kinesins. BTB-1 competes with ATP for Kif18A binding only when the motor-protein is associated with its pseudosubstrate microtubules. HeLa cells treated with BTB-1 accumulate in mitosis in a dose-dependent manner[1]. BTB-1 shows cell toxicity with an EC50 values of 35.8 μM. HeLa cells treated with 50 μM BTB-1 reveals severe defects in spindle morphology and chromosome alignment. Treatment with high concentrations of BTB-1 does not result in elongated spindles[2].